pgl2 luciferase reporter vector Search Results


pgl2 vector  (TaKaRa)
96
TaKaRa pgl2 vector
Pgl2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl2 basic vector dna  (Thermo Fisher)
99
Thermo Fisher pgl2 basic vector dna
Pgl2 Basic Vector Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector pgl2 empty control sv40 luc  (Addgene inc)
93
Addgene inc vector pgl2 empty control sv40 luc
Vector Pgl2 Empty Control Sv40 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl2-basic vector  (Promega)
90
Promega pgl2-basic vector
Pgl2 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl2 basic vector  (OriGene)
93
OriGene pgl2 basic vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Basic Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pgl2 basic vector - by Bioz Stars, 2026-02
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pgl2 basic vector  (Addgene inc)
92
Addgene inc pgl2 basic vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Basic Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
pgl2 basic vector - by Bioz Stars, 2026-02
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pgl2 vector  (Addgene inc)
93
Addgene inc pgl2 vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl2 vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
pgl2 vector - by Bioz Stars, 2026-02
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pgl2 reporter vector  (Addgene inc)
85
Addgene inc pgl2 reporter vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Reporter Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl2-297  (Geron Bio)
90
Geron Bio pgl2-297
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 297, supplied by Geron Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pgl2 basic vector  (New England Biolabs)
86
New England Biolabs pgl2 basic vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Basic Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl2 basic vector - by Bioz Stars, 2026-02
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pgl2 basic vector  (Thermo Fisher)
90
Thermo Fisher pgl2 basic vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Basic Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl2 basic vector/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pgl2 basic vector - by Bioz Stars, 2026-02
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Image Search Results


(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: Interaction of Positive Coactivator 4 with Histone 3.3 Protein is Essential for Transcriptional Activation of the Luteinizing Hormone Receptor Gene

doi: 10.1016/j.bbagrm.2018.09.002

Figure Lengend Snippet: (A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.

Article Snippet: Expression vectors and cell culture The reporter gene construct containing the LHR promoter was generated by cloning the human LHR gene promoter region (−176 to +1) into the SacI/BglII sites of the pGL2 basic vector [ 12 ]. pCMV6-PC4 was purchased from Origene (Rockville, MD) and used as PCR template for generation of constructs expressing PC4-Flag protein in MCF7 cells. p3XFLAG-PC4 vector was created by inserting PCR-amplified PC4 cDNA into the EcoRI and KpnI sites of the p3XFLAG-CMV-7.1 vector (Sigma) [ 11 ].

Techniques: Western Blot, Transfection, Activity Assay, Construct, Plasmid Preparation, Cell Culture